The existing diagnostic methods of identifying parasitic infections are based on outdated manual methods of microscope investigation of the content of the alimentary tract and of bile samples. These methods depend on qualifications of technicians, the quality of sample preparation, and the time of sample taking and are inadequate for correct diagnostics of these diseases in the acute and chronic phases and provide falsely negative results due to low infection rates.
In the framework of implementation of the complex project under the federal target program "Research and Development in Priority Science and Technology Areas”, a diagnostic kit has been developed and introduced into practice for real-time identification of opisthorchiasis, the most widespread human parasitic infection in the Siberian region. The kit's function is based on real-time identification of the parasite DNA in biological samples with the PCR method. This approach allows automation and simplification of the investigation procedure and significantly raises its reliability.
Currently, based on the existing scientific and engineering experience in the given area, the reagents are being developed to be included into the kit for diagnosing the following parasitic infections: ascariasis, opisthorchiasis, trichiniasis, giardiasis, anisakiasis, diphyllobothriasis, and trichocephaliasis.
To ensure complete acceptance of the already developed and being developed product in the clinical practice, we are planning to hold pilot tests of the product developed in Rospotrebnadzor institutions, to develop and get approvals for methodological recommendations for sanitary and epidemiological laboratories on promotion of new methods and upgrading the existing standards of providing medical care.